Anti-EV71 functionalized electrochemical probe development and analysis

Yi-Xiang Lu^1*, Chia-Yu Chang¹, Wen-Bin Fan², Jyh-Yuan Yang², Chia-Ching Chang^1,3

¹Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan
²Department of Health, Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan
³Institute of Physics, Academia Sinica, Taipei, Taiwan

* Presenter:Yi-Xiang Lu, email:oasis362573@gmail.com

Corresponding author: Chia-Ching Chang (ccchang01@faculty.nctu.edu.tw)

Enterovirus 71 (EV71) has become a global health issue that caused millions of infections every year. Conventional approaches such as cell culture and polymerase chain reaction (PCR) assay are time consuming. Therefore, a rapid, sensitive detection of enterovirus is required. Electrochemical impedance spectroscopy (EIS) is widely used for antigen-antibody interaction analysis. However, conventional antibody is not stable for EIS probe. We have synthsized an Enterovirus binding protein, which can conjugate with EIS electrode directly via self-assembly process. Moreover, this functionalized sensing probe can bind with EV71 sensitively and selectively within a few minutes. This platform is relatively stable to detect enterovirus and may reduce the risk of enterovirus outbreak.

Keywords: Enterovirus 71, biosensing, Electrochemical impedance spectroscopy