Anti-EV71 functionalized electrochemical probe development and analysis
Yi-Xiang Lu1*, Chia-Yu Chang1, Wen-Bin Fan2, Jyh-Yuan Yang2, Chia-Ching Chang1,3
1Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan
2Department of Health, Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan
3Institute of Physics, Academia Sinica, Taipei, Taiwan
* Presenter:Yi-Xiang Lu,
Corresponding author: Chia-Ching Chang (

Enterovirus 71 (EV71) has become a global health issue that caused millions of infections every year. Conventional approaches such as cell culture and polymerase chain reaction (PCR) assay are time consuming. Therefore, a rapid, sensitive detection of enterovirus is required. Electrochemical impedance spectroscopy (EIS) is widely used for antigen-antibody interaction analysis. However, conventional antibody is not stable for EIS probe. We have synthsized an Enterovirus binding protein, which can conjugate with EIS electrode directly via self-assembly process. Moreover, this functionalized sensing probe can bind with EV71 sensitively and selectively within a few minutes. This platform is relatively stable to detect enterovirus and may reduce the risk of enterovirus outbreak.

Keywords: Enterovirus 71, biosensing, Electrochemical impedance spectroscopy