Super-Resolution Architecture of Mammalian Centriole Distal Appendages Reveals Distinct Blade and Matrix Functional Components
Tony Yang1,2*, Weng Man Chong3, Won-Jing Wang4, Gregory Mazo5, Barbara Tanos6, Zhengmin Chen3, Thi Minh Nguyet Tran3, Yi-De Chen3, Rueyhung Roc Weng3, Chia-En Huang3, Wann-Neng Jane7, Meng-Fu Bryan Tsou5, Jung-Chi Liao3,8
1Department of Electrical Engineering, National Taiwan University, Taipei, Taiwan
2Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan
3Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, Taiwan
4Institute of Biochemistry and Molecular Biology, National Yang Ming University, Taipei, Taiwan
5Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
6Institute of Cancer Research, London, UK
7Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan
8Genome and Systems Biology Degree Program, National Taiwan University, Taipei, Taiwan
* Presenter:Tony Yang,
Distal appendages (DAPs) are nanoscale, pinwheel-like structures protruding from the distal end of the centriole that mediate membrane docking during ciliogenesis, marking the cilia base around the ciliary gate. Here, we determine a superresolved multiplex of 16 centriole-distal-end components. Surprisingly, rather than pinwheels, intact DAPs exhibit a cone-shaped architecture with components filling the space between each pinwheel blade, a new structural element we term the distal appendage matrix (DAM). Specifically, CEP83, CEP89, SCLT1, and CEP164 form the backbone of pinwheel blades, with CEP83 confined at the root and CEP164 extending to the tip near the membrane-docking site. By contrast, FBF1 marks the distal end of the DAM near the ciliary membrane. Strikingly, unlike CEP164 which is essential for ciliogenesis, FBF1 is required for ciliary gating of transmembrane proteins, revealing DAPs as an essential component of the ciliary gate. Our findings redefine both the structure and function of DAPs.

Keywords: super-resolution, distal appendage, centriole