Aluminum Nanoslit Arrays with a Commercial Scanner and Intensity Self-Referencing Analysis for High-Throughput Cellular Adhesion Analysis
Kuang-Li Lee1*, Hsien-San Hou1, Ji-Yen Cheng1, Pei-Kuen Wei1
1Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan
* Presenter:Kuang-Li Lee,
Development of high-throughput and user-friendly living cell sensing platform can benefit to cell biological research, drug discovery and development. A metallic nanostructure-based surface plasmon resonance (SPR) imaging platform, composed of a commercial transmission-type scanner, aluminum nanostructured-based SPR chips and self-referenced two-color analysis, is proposed for label-free and real-time cellular adhesion analysis. We demonstrated 25 sensing wells with a detectable depth of 600 nm and refractive index resolution of ~ 5 x 10-5. Compared to conventional intensity analysis, the resolution is improved by a factor of 5.6 using self-referenced two-color analysis. The sensing platform was applied for the kinetic studies of attachment and detachment of living cells under various doses of drugs. The results show that this sensing platform is capable of label-free and high-throughput detection of cell adhesion changes at the early stage and before the cell morphological change.

Keywords: Surface plasmon resonance imaging (SPRi), Nanostructures, Biochips, Cellular adhesion, Cytotoxicity